HSPA5/GRP78/BiP Antibody

• Catalog Number: orb1537563
• Category: Antibodies
• Description: HSPA5/GRP78/BiP Antibody
• Target: HSPA5 / GRP78 / BiP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-ESHQDADIEDFK, from the internal region (near C-Terminus) of the protein sequence according to NP_005338.1.
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: IF (5 µg/ml), IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 µg/ml)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA5 / GRP78 / BiP antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

HSPA5 / GRP78 / BiP antibody (0.3 µg/ml) staining of Mouse Liver (A) and Testes (B) and (0.5 ug/ml) Rat Brain (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.