HSP90 alpha Rabbit Polyclonal Antibody

SKU: orb157592

Description

HSP90 alpha Rabbit Polyclonal Antibody

Research Area

Cancer Research, Signal Transduction, Tumor Biomarkers

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSP90 alpha (401-500/732aa)
• Target: HSP90AA1
• Molecular Weight: 81 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP-90 alpha; HSP 86; HSP 86; Renal carcinoma antigen NY REN 38; Heat shock 86 kDa; Heat shock 90kDa protein 1 alpha; Heat shock protein 90kDa alpha(cytosolic) class A member 1; heat shock protein 90kDa alpha(cytosolic), class A member 2; Heat shock protein HSP 90-alpha; HS90A_HUMAN; HSP 86; HSP86; Hsp89; HSP89A; HSP90A; HSP90AA1; HSP90ALPHA; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL3; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP90 alpha) polyclonal Antibody, Unconjugated (orb157592) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Polyclonal Antibody, Unconjugated (orb157592) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, 293T KO HSP90 alpha (Human) Cell Lysate at 30 ug, Primary: Anti-HSP90 alpha (orb157592) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 77 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Human U251 cell lysates, Lane 4: Human Molt-4 cell lysates, Primary: Anti-HSP90 alpha (orb157592) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 90 kD.

Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-HSP90 alpha (orb157592) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 98 kD.

Quick Database Links

Gene Symbol

HSP90AA1

UniProt

P07900