Horse Immunoglobulin M (IgM) ELISA Kit

SKU: orb782753

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin M(IgM).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IgM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Equine
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 µg/mL
• Sensitivity: 0.71 µg/mL
• Concentration: 2000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGHM ELISA Kit, Immunoglobulin Heavy Constant Mu ELISA Kit, Ig mu chain C region ELISA Kit