Hoechst 33342 Staining Kit

• Catalog Number: orb219886
• Category: Tools
• Description: Hoechst 33342 Staining Kit
• Tested applications: ICC, IF
• Storage: Store at -20°C for one year.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).