Hoechst 33258 trihydrochloride

SKU: orb1303223

Description

Hoechst 33258 trihydrochloride is a fluorescent benzimidazole derivative that binds preferentially to AT-rich DNA sequences. This property makes it a vital tool for DNA quantification, chromosome staining, and cell cycle analysis in both live and fixed cells for in vitro research, while its anti-mitotic activity is also studied.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 23491-45-4
• MW: 533.9
• Purity: 99.53% (May vary between batches)
• Formula: C₂₅H₂₄N₆O·3HCl
• SMILES: CN1CCN(c(cc2)cc3c2nc(c4cc5c(N/C(N5)=C6C=CC(C=C\6)=O)cc4)[nH]3)CC1.Cl.Cl.Cl
• Target: Parasite
• Solubility: DMSO:6.58 mg/mL (12.32 mM)

Bioactivity

• Target IC50:
  U-937 cells:15.42±2.16 μM (IC50)|HeLa cells:51.31±4.56 μM (IC50)|HL-60 cells:32.43±3.27 μM (IC50)
• Cell Research:
  Solution preparation a. Preparation of stock solution: Dissolve 10 mg in 5 mL DMSO; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of Hoechst working solution Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/mL; (Select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL; b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes; c. Centrifuge at 400 g for 3-4 minutes at 4℃, and discard the supernatant; d. Wash twice with PBS, 5 minutes each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 3-10 minutes; d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H 33258, H 33258 trihydrochloride, H 33258 Trihydrochloride, H33258, H-33258, H33258 Trihydrochloride, H-33258 Trihydrochloride, Hoechst 33258, HOE 33258, Hoechst 33258 trihydrochloride, Hoechst 33258 Trihydrochloride, HOE33258, HOE-33258, inhibit, Inhibitor, Parasite, Bisbenzimide, bisBenzimide H 33258, bisBenzimide H 33258 Trihydrochloride, bisBenzimide H 33258 trihydrochloride, bisBenzimide H33258, bisBenzimide H-33258, bisBenzimide H33258 Trihydrochloride, bisBenzimide H-33258 Trihydrochloride, Pibenzimol

Chemical structure of Hoechst 33258 trihydrochloride

Compound Identifiers

PubChem CID

31953