Hoechst 33258 analog 2

SKU: orb1307183

Description

Hoechst 33258 analog 2 is a cell-permeable, blue-fluorescent dye for DNA staining. It is widely used in cell biology for chromosome visualization, cell cycle analysis, and viability assays in both live and fixed cells for in vitro research applications.

Research Area

Cell Biology

Key Properties

• CAS Number: 23491-54-5
• MW: 422.52
• Purity: 98.4%
• Formula: C₂₆H₂₆N₆
• SMILES: CN1CCN(CC1)c1ccc2nc([nH]c2c1)-c1ccc2nc([nH]c2c1)-c1cccc(C)c1
• Target: Autophagy,Others
• Solubility: DMSO:60 mg/mL (142.01 mM);H2O:< 0.1 mg/mL (insoluble)

Bioactivity

• In Vitro:
  The dyes Hoechst 33258 and Hoechst 33342 ar the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nM, and both emit blue/cyan fluorescent light around an emission maximum at 461 nM. Unbound dye has its maximum fluorescence emission in the 510-540 nM range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. concentration can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least Six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C . Althoug the dyes can bind to All nucleic acids, AT-rich double-st anded DNA st and enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells.
• Cell Research:
  I. Solution preparation 1. Stock solution preparation: Dissolve Hoechst 33258 analog 2 in DMSO or water to prepare a storage solution with a concentration of 1-10 mM. 2. Working solution preparation: Dilute to the appropriate concentration with PBS/DMEM/H2O before use the common working concentration is 1-10 μM, bu the specific concentration should be optimized according to Experimental requirements. II. Cell staining 1. Cell culture: Culture cells in appropriate culture dishes to ensure tha the cells Each an appropriate density (e.g., 70%-80% confluence). 2. Staining: Ad the working concentration of Hoechst 33258 analog 2 to the cell culture medium the common working concentration is 1-10 µM, bu the specific concentration can be adjusted according to Experimental needs. 3. Incubate cells at room temperature or 37°C for 10-30 minutes the specific time can be adjusted according to Experimental requirements. 4. Wash cells: After staining, wash cells with PBS (phosphate buffered saline) to remove unbound dye and prevent nonspecific staining. 5. Fluorescence detection: Hoechst 33258 analog 2 emits blue fluorescence under UV light (360 nM) excitation, with an emission wavelength of 460 nM the dye binds specifically to DNA, and its fluorescence can be detected by a fluorescence microscope or flow cytometer equipped with appropriate filters. Notes: 1. Photosensitivity: Hoechst dye is light-sensitive, and long-term exposure to strong light may affect its fluorescence intensity. Therefore, it is recommended to andle in a low-light envir nMent and avoid unnecessary light exposure during storage and operation. 3. Cytotoxicity: At high concentration , Hoechst dye may be cytotoxic to cells. Therefore the concentration needs to be optimized to reduce possible cell damage.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

inhibit, Hoechst 33258 analog 2, Inhibitor

Chemical structure of Hoechst 33258 analog 2

Compound Identifiers

PubChem CID

44386468