HOE-S 785026 trihydrochloride

SKU: orb1706359

Description

HOE-S 785026 trihydrochloride is a cell-permeable, nucleic acid-binding dye that selectively targets the minor groove of A/T-rich double-stranded DNA, resulting in enhanced fluorescence. Its pH-dependent fluorescence intensity makes it suitable for live-cell imaging and nucleic acid staining in both in vitro and in vivo research applications.

Key Properties

• CAS Number: 2320308-12-9
• MW: 533.88
• Purity: 99.65%
• Formula: C₂₅H₂₇Cl₃N₆O
• SMILES: OC1=CC=CC(C2=NC3=CC(C4=NC5=CC(N6CCN(C)CC6)=CC=C5N4)=CC=C3N2)=C1.[H]Cl.[H]Cl.[H]Cl
• Target: Others
• Solubility: H2O:47.3 mg/mL (88.6 mM)

Bioactivity

• Cell Research:
  Instructions I. Solution preparation 1. Preparation of mother solution: Dissolve HOE-S 785026 trihydrochloride in anhydrous DMSO to prepare a 1 mg/mL mother solution. To ensure complete dissolution, the mother solution can be warmed, but high temperature should be avoided to prevent degradation. Note: When storing the mother solution, be sure to seal it and store it at -20°C away from light. 2. Preparation of working solution: Dilute the mother solution to the required concentration in an appropriate proportion, usually using a working concentration of 5-10 μg/mL. Use serum-free cell culture medium, PBS or other suitable buffer for dilution. The specific concentration can be adjusted according to experimental needs. II. Operation steps 1. Cell staining: Adherent cells: First culture the cells in a culture dish to an appropriate density, add the working solution, and incubate at room temperature for 15-20 minutes. Suspended cells: Collect the cells by centrifugation, remove the culture medium, add the working solution, and incubate at room temperature for 10-15 minutes. During the incubation process, ensure that the cells are evenly exposed to the dye solution. 2. Washing steps: 1) After incubation, wash the cells three times with PBS or culture medium, gently centrifuging each time to remove excess dye. 2) Resuspend the cells with fresh culture medium and prepare for fluorescence detection. 3. Fluorescence observation: Use a fluorescence microscope at a wavelength of 350-370 nm for excitation and an emission wavelength of approximately 460-470 nm to observe the staining results.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

132869-83-1, 132869-83-1 free base, Dye reagent, meta-Hoechst, meta-Hoechst trihydrochloride, Meta-Hoechst trihydrochloride, meta-Hoechst Trihydrochloride, HOE-S 785026, HOE-S 785026 Trihydrochloride, HOE-S 785026 trihydrochloride (132869-83-1 free base), HOE-S785026, HOE-S-785026, HOE-S785026 Trihydrochloride, HOE-S-785026 Trihydrochloride

Compound Identifiers

PubChem CID

131704504