HIST1H3D Antibody

SKU: orb1253750

Description

HIST1H3D Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Histone H3.1 (Phospho-Ser10) antibody was raised against a peptide sequence around phosphorylation site of serine 10 (R-K-S (p) -T-G) derived from Human Histone H3.1.
• Target: HIST1H3D
• Molecular Weight: 17 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h

Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using Histone H3.1 (Phospho-Ser10).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (Phospho-Ser10). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Phospho-Ser10).

Quick Database Links

Gene Symbol

HIST1H3D

UniProt

P68431

RefSeq (Protein):NP_003521.2