Histone H3 (Acetyl Lys23) rabbit pAb

• Catalog Number: orb763964
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (Acetyl Lys23)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23. AA range:-9-40
• MW: 17
• Tested applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H3A antibody, anti-H3FA antibody, anti-H
• Note: For research use only

Western Blot analysis of various cells using Acetyl-Histone H3 was diluted at 1:20000.

Western Blot analysis of L929 cells using Acetyl-Histone H3 was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.