Recombinant His Tag Antibody / Histidine

SKU: orb1151688

Description

Mouse monoclonal antibody to His Tag

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: All
• Application Notes:
  Optimal dilution of the recombinant His Tag antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: r6HIS/6423
• Immunogen: Hexa-histidine tagged human CD70 recombinant protein was used as the immunogen for the recombinant His Tag antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of a His-tagged protein using recombinant His Tag antibody (clone r6HIS/6423).

SDS-PAGE analysis of purified, BSA-free recombinant His Tag antibody (clone r6HIS/6423) as confirmation of integrity and purity.