HighYield T7 Cy3 RNA Labeling Kit (UTP-based)

SKU: orb611239

Description

HighYield T7 Cy3 RNA Labeling Kit (UTP-based) is designed to produce randomly Cy3-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY3 (also known as Cy3-17-UTP from GE Healthcare) is effi-ciently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Poly-merase Mix. 35% UTP-X-Cy3 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy3/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy3-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-X-Cy3 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy3).

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1, cm-1 (Tris-HCl pH 7.5).

Key Properties

• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=C\C=C\C1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Preparation of randomly Cy3-modified RNA probes by in vitro transcription with UTP-X-CY3

Compound Identifiers

PubChem CID

176417999