GST Antibody Fluorescein Conjugated

SKU: orb345837

Description

GST antibody (FITC)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:60,000, WB: 1:2,000 - 1:10,000
• Application Notes:
  FITC conjugated GST Antibody has been tested by western blot and is ideal for microscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
• Purity: FITC conjugated GST Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Conjugation: FITC

Storage & Handling

• Storage: Store FITC conjugated GST Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-GST antibody fluorescein conjugation, FITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Western Blot of Rabbit anti-GST Fluorescein Conjugated Antibody. Lane 1: GST. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GST. Other band(s): None.