Granzyme B (NK/T-Cell Lymphoma Marker) Antibody

• Catalog Number: orb751163
• Category: Antibodies
• Description: Mouse monoclonal antibody against GZMB conjugated to Biotin
• Target: GZMB
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein A
• Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)
• UniProt ID: P10144
• MW: 29-32kDa
• Tested applications: IHC, WB
• Dilution range: Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: A431 cells. Human tonsil, spleen or Hodgkin's Lymphoma tissue (IHC).
• Clone Number: GZMB/3014
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Cathepsin G-like 1 antibody, anti-CCPI antibo
• Research Area: Cardiovascular Research, Cell Biology, Signal Tran
• Note: For research use only
• Entrez: 3002
• Expiration Date: 12 months from date of receipt.

Analysis of Protein Array containing >19000 full-length human proteins using Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human spleen stained with Biotinylated Granzyme B Mouse Monoclonal Antibody (GZMB/3014).

Formalin-fixed, paraffin-embedded human Spleen stained with Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014).

Formalin-fixed, paraffin-embedded human Tonsil stained with Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014).

SDS-PAGE Analysis Purified Granzyme B Mouse Monoclonal Antibody (GZMB/3014). Confirmation of Integrity and Purity of Antibody.