USP28 Antibody

SKU: orb19956

Description

Goat polyclonal antibody to USP28

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:64000, WB: 0.2 μg/ml, IHC-P: 2.5-3.8µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: USP28
• Protein Sequence: SVELKHYIQEDN
• Molecular Weight: 122
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti USP28 antibody, anti ubiquitin specific peptidase 28 antibody, anti KIAA1515 antibody, anti ubiquitin specific protease 28 antibody

Immunohistochemical staining of Human Testis using USP28 antibody

Quick Database Links

Gene Symbol

USP28

NCBI Gene ID

57646