UBE2I Antibody

• Catalog Number: orb18871
• Category: Antibodies
• Description: Goat polyclonal antibody to UBE2I
• Target: UBE2I
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SGIALSRLAQERK
• RRID: AB_10749782
• MW: 18.0; 18.0; 18.0; 18.0
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1ug/ml.WB: Approx 17kDa band observed in Human Kidney, Lung and Testis lysates and in Mouse and Rat Heart, Spleen and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended concentration: 0.1-0.3 μg/ml
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UBE2I antibody, anti ubiquitin-conjugating en
• Note: For research use only
• Entrez: 7329
• Expiration Date: 12 months from date of receipt.

Western blot analysis of staining of Human Kidney lysate using UBE2I antibody.

orb18871 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Western blot analysis of Rat Ovary lysate using UBE2I antibody