PRMT5 Antibody

SKU: orb20273

Description

Goat polyclonal to PRMT5

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 70kDa band observed in Jurkat lysates (calculated MW of 72.7kDa and 71.3kDa according to NP_006100.2; NP_001034708.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PRMT5
• Protein Sequence: RACREKDRDPEAQ
• Molecular Weight: 72.7; 71.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PRMT5 antibody, anti protein arginine methyltransferase 5 antibody, anti HRMT1L5 antibody, anti IBP72 antibody, anti JBP1 antibody, anti SKB1 antibody, anti SKB1Hs antibody, anti HMT1 hnRNP methyltransferase-like 5 antibody, anti SKB1 homolog antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

PRMT5

NCBI Gene ID

10419