NKD2 Antibody

SKU: orb19684

Description

Goat polyclonal antibody to NKD2

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA: 1:32000, WB: 0.1 μg/ml
• Predicted Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 80kDa, 38kDa and 26kDa in Mouse Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.5kDa according to Mouse NP_082462.2 and of 50.1kDa according to Human NP_149111.1. All the detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NKD2
• Molecular Weight: 50.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NKD2 antibody, anti naked cuticle homolog 2 (Drosophila) antibody, anti Dvl-binding protein NKD2 antibody, anti naked cuticle homolog 2 antibody, anti naked cuticle-2 antibody

Quick Database Links

Gene Symbol

NKD2

NCBI Gene ID

85409