NEFL (aa330-343) Antibody

SKU: orb1796

Description

Goat polyclonal antibody to NEFL

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 68-70kDa band observed in lysates of Neuroblastoma cell line KELLY (calculated MW of 61.5kDa according to NP_006149.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NEFL (aa330-343)
• Protein Sequence: EKQLQELEDKQNAD
• Molecular Weight: 61.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFL, neurofilament, light polypeptide, CMT1F, CMT2E, NF-L, NF68, NFL, PPP1R110, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptid

Western blot analysis of KELLY lysate using NEFL antibody

Quick Database Links

Gene Symbol

NEFL (aa330-343)

NCBI Gene ID

4747