Goat KT3 Tag Antibody

SKU: orb1530850

Description

KT3 Tag Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:30,000; IP - 1 - 4 µg/mg lysate; ICC - 1:100 - 1:400; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
• Application Notes:
  Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Target: KT3 Tag
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection of KT3-tagged protein by western blot.Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-KT3 antibody orb1530850 at 0.2 µg/ml (1:5, 000).

Quick Database Links

Gene Symbol

KT3 Tag