Glycophorin A Antibody

SKU: orb749548

Description

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Glycophorin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: GYPA/280
• Immunogen: Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC-P: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Glycophorin A antibody (clone GYPA/280).

IHC-P: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Glycophorin A antibody (clone GYPA/280).

IHC-P: Formalin-fixed, paraffin-embedded human placenta stained with Glycophorin A antibody (clone GYPA/280).

Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone GYPA/280). Expected molecular weight: routinely observed at ~16/38 kDa.

SDS-PAGE analysis of purified, BSA-free Glycophorin A antibody (clone GYPA/280) as confirmation of integrity and purity.

Quick Database Links

UniProt

P02724