Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody

SKU: orb2563070

Description

Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 3A14
• Immunogen: A synthesized peptide derived from human GPI (1-64/558aa)
• Target: GPI
• Molecular Weight: 63 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMF; CNSHA4; GNPI; NLK; PGI; PHI; SA-36; SA36; Gpi; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; Gpi1s; MF; NK; NK/GPI; Org; Gpi1; G6PI_HUMAN; Autocrine motility factor (AMF); Neuroleukin (NLK); Phosphoglucose isomerase (PGI); Phosphohexose isomerase (PHI); Sperm antigen 36 (SA-36); 5.3.1.9; G6PI_MOUSE; G6PI_RAT;

25 ug total protein per lane of various lysates probed with Glucose 6 phosphate isomerase monoclonal antibody, unconjugated (orb2563070) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (orb2563070) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

GPI