Recombinant GFAP Antibody / Rabbit Monoclonal

SKU: orb2640615

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: ASTRO/1974R
• Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of frozen human cerebral cortex tissue with recombinant GFAP antibody (blue, clone ASTRO/1974R) and Histone H1 antibody (magenta, clone HH1/957).

IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone ASTRO/1974R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone ASTRO/1974R) as confirmation of integrity and purity.

Flow cytometry testing of fixed human T98G cells with recombinant GFAP antibody (clone ASTRO/1974R); Red = isotype control, Blue = recombinant GFAP antibody.

Quick Database Links

UniProt

P14136