General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

• Catalog Number: orb1806551
• Category: Assays and Kits
• Description: General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit
• Target: cAMP
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Adenosine Monophosphate(cAMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Adenosine Monophosphate(cAMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Adenosine Monophosphate(cAMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 20000 pg/mL
• Range: 312.5-20000 pg/mL
• Sensitivity: 92.5 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adeno
• Note: For research use only