General Androsterone (ADT) ELISA Kit

• Catalog Number: orb1806536
• Category: Assays and Kits
• Description: General Androsterone (ADT) ELISA Kit
• Target: ADT
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androsterone(ADT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androsterone(ADT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androsterone(ADT) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 90000pg/mL
• Range: 1406.25-90000pg/mL
• Sensitivity: 144 pg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone
• Research Area: Metabolism Research, Stem Cell & Developmental Bio
• Note: For research use only
• Expiration Date: Please enquire.