General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

• Catalog Number: orb1806543
• Category: Assays and Kits
• Description: General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
• Target: 8-epi-PGF2a
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 2000pg/mL
• Range: 31.25-2000pg/mL
• Sensitivity: 9.55 pg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: 8-EpiPGF2-α; 8epi-PGF2a
• Research Area: Gastroenterology & Hepatology, Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.