General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

• Catalog Number: orb1806521
• Category: Assays and Kits
• Description: General 17-Hydroxyprogesterone (17-OHP) ELISA Kit
• Target: 17-OHP
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 17-Hydroxyprogesterone(17-OHP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Hydroxyprogesterone(17-OHP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Hydroxyprogesterone(17-OHP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 20000 pg/mL
• Range: 312.5-20000 pg/mL
• Sensitivity: 87.3 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: 17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogest
• Note: For research use only