Gamma Tubulin Mouse Monoclonal Antibody

SKU: orb499656

Description

Gamma Tubulin Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 8D11
• Immunogen: KLH conjugated synthetic peptide derived from human Gamma Tubulin
• Target: TUBG1
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Centrosome Marker; gamma 1 tubulin; gamma tubulin; gamma-tubulin; gamma 2 tubulin; gamma Tubulin 1; gamma Tubulin 2; gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; gamma-1-tubulin; gamma-tubulin complex component 1.

Blank control (black line): Hela. Primary Antibody (green line): Mouse Anti-Gamma Tubulin antibody (orb499656), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (orb499656) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (orb499656) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Cerebellum tissue lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human A549 cell lysates, Primary: Anti-Gamma Tubulin (orb499656) at 1/3000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

TUBG1

UniProt

P23258