Galectin 3 Mouse Monoclonal Antibody

• Catalog Number: orb526490
• Category: Antibodies
• Description: Galectin 3 Mouse Monoclonal Antibody
• Target: LGALS3
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: Recombinant human Galectin-3 full length protein
• UniProt ID: P17931
• MW: 29 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 8A2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LEG3_HUMAN; Galectin-3; LGALS3; Gal-3; 35 kDa lect
• Research Area: Cell Biology, Cell Differentiation, Nuclear Protei
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2 Galectin 3) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, U251 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.