FTO Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499264
• Category: Antibodies
• Description: FTO Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 19H6
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FTO
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: FTO
• UniProt ID: Q9C0B1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha-ketoglutarate-dependent dioxygenase FTO; Fat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (orb1499264) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human SW480 cell lysates, Primary: Anti-FTO (orb1499264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kDa, Observed band size: 62 kDa.