Frizzled 8 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563051
• Category: Antibodies
• Description: Frizzled 8 Recombinant Rabbit Monoclonal Antibody
• Target: FZD8
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human Frizzled 8 (640-694/694aa)
• UniProt ID: Q9H461
• MW: 70 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IF=1:100-500, IHC-F=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 15G15
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Frizzled 8 seven transmembrane spanning receptor;
• Research Area: G Protein-Coupled Receptors, GPCR Signaling Pathwa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Uterus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Frizzled 8 Monoclonal Antibody, Unconjugated (orb2563051) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.