Fluorescein-12-dCTP

SKU: orb533142

Description

Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/70-50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 991.68 g/mol; Detected MW: 991.15 g/mol
• Purity: ≥ 95% (HPLC)
• Formula: C₃₉H₄₀N₅O₂₀P₃
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)C(=O)O)N)O.[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)C(=O)O)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)N)O

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only