FKBP52 Antibody (APC)

SKU: orb147210

Description

Mouse monoclonal to FKBP52 (APC). Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR co-chaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors..

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution range: WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg)
• Reactivity: Canine, Hamster, Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: Hi52C
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP52
• Target: FKBP52
• Molecular Weight: 52kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

FK506 binding protein 4, FKBP4, FKBP59, HBI, HSP56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein

Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: prostate tissue (ductal epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 μg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 μg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red).

Quick Database Links

Gene Symbol

FKBP52

UniProt

Q02790

NCBI Gene ID

2288

RefSeq (Protein):NP_002005.1