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F(ab')2 Rat IgG Fc Antibody Rhodamine Conjugated Pre-Adsorbed
Catalog Number: orb348332
| Catalog Number | orb348332 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Rat IgG F(c) antibody (TRITC) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Isotype | IgG F(ab')2 |
| Conjugation | TRITC |
| Reactivity | Rat |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins. |
| Immunogen | Rat IgG F(c) fragment |
| Tested applications | FC, FLISA, IF |
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Application notes | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | Goat F(ab')2 Anti-Rat IgG F(c) Rhodamine Conjugate Read more... |
| Note | For research use only |
Expression of APN in brain parenchyma vessels. Adult mice were perfused with 4% paraformaldehyde and brain sections incubated with the antibody ER-BMDM1 or a mixture of ER-BMDM1 and antiserum to the VW factor as indicated in Section 2. (A) Single labelling with ER- Ž. BMDM1.
