F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb348245
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: FITC
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Immunogen: Mouse IgG whole molecule
• Tested applications: ELISA, FC, FLISA, IF, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat F(ab')2 Anti-Mouse IgG Antibody Fluorescein C
• Note: For research use only

Western Blot of Goat anti-Mouse IgG Fluorescein Conjugated Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Mouse IgG. Other band(s): None.