ER Antibody / Estrogen Receptor alpha

SKU: orb385819

Description

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NR3Ga-3
• Immunogen: Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS testing of human MCF-7 cells: Black = cells alone; Green = isotype control; Red = PE conjugated ER antibody.

IHC testing of human breast carcinoma stained with Estrogen receptor antibody (NR3Ga-3). FFPE testing requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTAfor 10-20 min and cooling at RT for 20 minutes.

Western blot testing of MCF-7 cell lysate with ER antibody (clone NR3Ga-3). Predicted molecular weight of ER alpha: ~66 kDa.