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Description
Research Area
Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | IHC - 1:2000, WB - 1:1000, IHC-P - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b,k |
| Clone No. | B491EV39X3X3 |
| Immunogen | This ENOG monoclonal antibody is generated from mouse immunized with ENOG recombinant protein. |
| Target | ENO2 |
| Molecular Weight | 47269 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
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All lanes: Anti-ENOG at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: CEM whole cell lysate. Lane 3: U-251 MG whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
ENOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ENOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Immunohistochemical analysis of paraffin-embedded Human pancreas section using Pink1. diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the Secondary followed by DAB staining.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001966.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ENOG Antibody (orb1939350)
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