E-Cadherin Antibody / CDH1

• Catalog Number: orb534005
• Category: Antibodies
• Description: Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.
• UniProt ID: P12830
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: ECD1-3R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).

Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone ECD1-3R). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Formalin-fixed, paraffin-embedded human cervix stained with recombinant E-Cadherin antibody (clone ECD1-3R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R); Red = isotype control, Blue = recombinant E-Cadherin antibody.