DNA Ligase IV Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704348
• Category: Antibodies
• Description: DNA Ligase IV Recombinant Rabbit Monoclonal Antibody
• Target: LIG4
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human DNA Ligase IV
• UniProt ID: P49917
• MW: 104 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 2D9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LIG4; DNA ligase IV ATP dependent; DNA joinase; DN
• Research Area: Molecular Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DNA Ligase IV) Monoclonal Antibody, Unconjugated (orb704348) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DNA Ligase IV) Monoclonal Antibody, Unconjugated (orb704348) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human Jurkat cell lysates, Lane 3: Human HT-1080 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human Ramos cell lysates, Lane 6: Human HepG2 cell lysates, Primary: Anti-DNA Ligase IV (orb704348) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 104 kDa, Observed band size: 104 kDa.