Desmin Antibody

SKU: orb385939

Description

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 0.5-1ug/10^6 cells,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the Desmin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: DES/1711
• Immunogen: Recombinant human Desmin was used as the immunogen for the Desmin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human uterus with Desmin antibody (clone DES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Desmin Antibody (clone DES/1711). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P17661