Recombinant Cyclin E1 Antibody / CCNE1

SKU: orb2637008

Description

Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Cyclin E1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rCCNE1/4936
• Immunogen: A portion of amino acids 10-176 was used as the immunogen for the Cyclin E1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936). Predicted molecular weight ~47 kDa.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant Cyclin E antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant Cyclin E1 antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cyclin E1 antibody (clone rCCNE1/4936) as confirmation of integrity and purity.

Quick Database Links

UniProt

P24864