Cyclin D3 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172536
• Category: Antibodies
• Description: Cyclin D3 Recombinant Mouse Monoclonal Antibody
• Target: CCND3
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG1, Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Tested applications: ICC, WB
• Dilution range: WB=1:500-1000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: G12E5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cell Biology, Cell Cycle, Cyclin D family, Cyclins
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: BRL, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 33 kDa, Observed MW: 33 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, Daudi, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 33 kDa, Observed MW: 33 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172536.