Cyclin B1 Antibody

• Catalog Number: orb1259192
• Category: Antibodies
• Description: Cyclin B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: CCNB1
• UniProt ID: P14635
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CCNB1, CCNB
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (orb1259192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (orb1259192). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (orb1259192) at a dilution of 1:1000.