Cyclin B1 Antibody

SKU: orb749862

Description

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC-P
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Cyclin B1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CCNB1/1098
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cyclin B1 antibody (CCNB1/1098).

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin B1 antibody.

Immunofluorescent staining of human MCF7 cells with Cyclin B1 antibody (clone CCNB1/1098, red), phalloidin (actin, green) and Hoechst (nuclei, blue).

SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone CCNB1/1098) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14635