Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat]

• Catalog Number: orb1675134
• Category: Antibodies
• Description: Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rabbit
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Immunogen: Rabbit IgG, whole molecule
• Tested applications: FACS
• Application notes: FLOW CYTOMETRY 1:200 - 1:400 IF MICROSCOPY 1:100 - 1:1,000 OTHER APPLICATIONS User Optimized Pre-Conjugation ELISA Data Minimum ELISA Endpoint Dilution: 1:10,000 Actual: 1:41,000 Disposition: PASS
• Antibody Type: Secondary Antibody
• Storage: For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Note: For research use only