CTNNB1 Antibody (C-term)

• Catalog Number: orb1925519
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 691CT11.2.1
• Tested applications: IF, WB
• Predicted Reactivity: Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Dilution range: IF: 1:25, WB: 1:4000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 85497 Da
• Target: This CTNNB1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 687-721 amino acids from the C-terminal region of human CTNNB1.
• UniProt ID: P35222
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-CTNNB1 Antibody (C-term) at 1:4000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: COS-7 whole cell lysate. Lane 4: C6 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 98 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling CTNNB1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).