Crystallin Alpha B Antibody

SKU: orb2310020

Description

Crystallin Alpha B Antibody

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: CRYAB/4666
• Immunogen: Recombinant fragment (around aa1-175) of human CRYAB protein (exact sequence is proprietary)
• Target: CRYAB
• Source/Expression System: Bioreactor
• Molecular Weight: Predicted: 20kDa; Observed: 22-30kDa
• Purification: Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

AACRYA; Alpha B crystallin; Alpha-crystallin B chain; CRYA2; CTTP2; Heat shock 20 kD like protein; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY REN 27; Rosenthal fiber component

Analysis of Protein Array containing more than 19000 full-length human proteins using Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4666). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human kidney stained with Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4666). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4666). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of HeLa cell lysate using Crystallin Alpha B Mouse Monoclonal Antibody (CRYAB/4666).

Western blot analysis of Human Kidney lysates using CRYAB Mouse Monoclonal Antibody (CRYAB/4666).

Quick Database Links

Gene Symbol

CRYAB

UniProt

P02511