COH-SR4

SKU: orb1298671

Description

COH-SR4 is a mitochondrial oxidative phosphorylation uncoupler that inhibits hepatocellular carcinoma cell proliferation by modulating the AMPK-mTOR signaling pathway. It serves as a research tool for studying cancer metabolism and has demonstrated anti-proliferative effects in in vitro models like HepG2 cells.

Research Area

Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 73439-19-7
• MW: 350.03
• Purity: 99.67% (May vary between batches)
• Formula: C₁₃H₈Cl₄N₂O
• SMILES: Clc1cc(Cl)cc(NC(=O)Nc2cc(Cl)cc(Cl)c2)c1
• Target: AMPK
• Solubility: DMSO:4 mg/mL (11.43 mM)

Bioactivity

• In Vivo:
  SR4 is a novel mitochondrial uncoupler with anti-obesity and anti-diabetic properties. SR4 increased oxygen consumption, dissipated mitochondrial membrane potential, induced mitochondrial swelling, and decreased intracellular ATP in cultured cells and isolated liver mitochondria. Oral feeding of SR4 significantly reduced body weight gain, improved glycemic control and insulin resistance, and prevented dyslipidemia in both high-fat-diet (HFD) induced obese and diabetic db/db mice. SR4 treatment also decreased liver triglycerides and prevented hepatic steatosis in both animal models. Mitochondrial uncoupling of SR4 results to activation of AMP-activated protein kinase (AMPK), leading to the phosphorylation and inhibition of acetyl-CoA carboxylase (ACC). Gene analyses by RT-PCR showed SR4 significantly suppressed the mRNA expression of several lipogenic genes and gluconeogenic genes in the liver of HFD obese mice. RNA sequencing analysis showed that 642 genes were differentially expressed in liver of db/db mice after SR4 treatment (217 upregulated, 425 down-regulated). Gene ontology analysis by DAVID indicated SR4 upregulated amino acid metabolism and down-regulated lipid and fatty acid synthesis and glucose metabolism. These studies demonstrate that SR4 may be a promising compound for treatment of T2DM and obesity

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

leukemia, melanoma, Inhibitor, lung, inhibit, kinase, Mitochondria uncoupler SR4, AMP-activated protein kinase, anti-proliferative, AMP-activated, AMPK, anticancer, anti-adipogenic, cancers, breast, COH SR4, COHSR4, COH-SR4, COH-SR-4, COH-SR4 (Mitochondria uncoupler SR4), protein

Compound Identifiers

PubChem CID

595311