Cofilin 1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1172841

Description

Cofilin 1 Recombinant Rabbit Monoclonal Antibody

Research Area

Actin Binding Proteins, Actina, Axonal Guidance Proteins, Cell Adhesion, Cell Biology, Cytoskeleton, Neuroscience

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1:20-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: C5E4
• Target: CFL1
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: MCF-7, 3: 293T, 4: C6, 5: NIH/3T3, Protein loading quantity: 20 µg, Exposure time: 3 s, Predicted MW: 19 kDa, Observed MW: 19 kDa.

Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172841.

Quick Database Links

Gene Symbol

CFL1