CK20 (ready to use) Mouse Monoclonal Antibody

• Catalog Number: orb544674
• Category: Antibodies
• Description: CK20 (ready to use) Mouse Monoclonal Antibody
• Target: KRT20
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 1% rAlbumin and 0.02% Proclin300.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human CK20
• UniProt ID: P35900
• MW: 48 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Antibody Type: Primary Antibody
• Clone Number: 6A12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cytokeratin 20; CK 20; CK20; Cytokeratin20; K20; K
• Research Area: Cancer Research, Signal Transduction, Tumor Biomar
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (orb544674) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (orb544674) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.