Citric acid monohydrate

SKU: orb1220818

Description

Citric Acid Monohydrate is a tricarboxylic acid found in citrus fruits. Citric acid is used as an excipient in pharmaceutical preparations due to its antioxidant properties. It maintains stability of active ingredients and is used as a preservative.

Key Properties

• CAS Number: 5949-29-1
• MW: 210.14
• Purity: >98% (HPLC)
• Formula: C₆H₁₀O₈
• SMILES: O.OC(=O)CC(O)(CC(O)=O)C(O)=O
• Target: Others
• Solubility: In Vitro: DMSO : 100 mg/mL (475.87 mM)

Bioactivity

• In Vivo:
  Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.) significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver. Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.) induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes. Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) causes renal toxicity in mice. Animal model: 20 g male Kunming mice. Dosage: 120, 240, 480 mg/kg. Administration: i.p.; weekly for 3 weeks. Result: T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.
• In Vitro:
  Citric acid monohydrate (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner. Citric acid monohydrate (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner. Citric acid monohydrate (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl. Cell Viability Assay Cell line: HaCaT cells. Concentration: 0, 2.5, 5, 7.5, 10, 12.5 mM Incubation time: 24 h. Result: Inhibited the cell viability in a dose dependent manner. Cell Cycle Analysis Cell line: HaCaT cells. Concentration: 12.5 mM Incubation time: 0, 12, 24, 48, 72 h. Result: Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner. Western blot analysis. Cell line: HaCaT cells. Concentration: 12.5 mM Incubation time: 12, 24, 48 h. Result: Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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